Articles Accepted

RP - HPLC METHOD FOR THE DETERMINATION OF MEBEVERINE HYDROCHLORIDE IN TABLET DOSAGE FORMS EMPLOYING MS COMPATIBLE BUFFERS
by Dr. vijayalakshmi marella, 01 Feb 2019

A simple, precise, rapid, accurate and economic reverse phase high performance liquid chromatographic method has been developed for the estimation of mebeverine hydrochloride in bulk and tablet dosage form. The separation was achieved by using kinetex column (C18) (150×4.6mm, 5µ) and 15mM ammonium acetate and methanol in proportion of 30:70 v/v as mobile phase, at a flow rate of 1.0ml/min. The detection was carried out at 230nm.The retention time of mebeverine was found to be 3.4min.The limit of detection and limit of quantitation were found to be 0.005µg/ml and 0.016µg/ml respectively. The reliability of the proposed method was ascertained by evaluating various validation parameters like linearity, precision, accuracy and specificity according to ICH guidelines. The proposed method applies LC-MS compatible buffers which increase’s the life time of column. This method acts as a quality control tool for routine analysis of mebeverine hydrochloride in bulk and tablet dosage forms.

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November 2020
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