Articles Accepted

A VALIDATED NOVEL SPECTROSCOPIC METHOD FOR ESTIMATION OF TRIAMCINOLONE ACETONIDE IN NASAL SPRAY
by Mr. BHOOMIBEN PATEL, 22 Mar 2019

A simple, sensitive, accurate, precise and cost-effective pH independent spectrophotometric method has been developed for the estimation of Triamcinolone acetonide in nasal sprays. Estimation was carried out at an isosbestic point of drug solutions, Isosbestic point is measured by scanning equimolar solution of Triamcinolone acetonide separately in phosphate buffer pH 3.0, phosphate buffer pH 7.0 and phosphate buffer pH 10.0 at a concentration of 10 µg/ml. They were scanned in the wavelength range of 200-400 nm. The isosbestic point is found to be 230.0 nm in acidic, basic and neutral condition; hence pH independent wavelength 230 nm is used for estimation of Triamcinolone acetonide. The method observed linear response in the concentration range of 3-18 μg/ml at 230 nm. Developed method has been validated accordance to ICH guideline Q2R1 for several parameters like linearity, precision, accuracy, limit of detection and limit of quantification. The developed and validated analytical method is successfully applied to the estimation of Triamcinolone acetonide in marketed nasal spray formulations.

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November 2020
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