Articles Accepted

DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR THE ESTIMATION OF VOGLIBOSE IN BULK AND TABLET DOSAGE FORMS
by Dr. MALLIKHARJUNA RAO NAGASARAPU, 26 Mar 2019

A simple, fast, specific and precise High Performance Thin Layer Chromatographic method (HPTLC) has been developed for estimation of voglibose in bulk and pharmaceutical formulations. The chromatographic separation was carried out on precoated silica gel 60F254 aluminum plates using a mixture of acetonitrile: methanol: ammonia (15:4:0.1% v/v/v) as mobile phase and densitometric evaluation of spots was carried out at 284nm using Camag TLC scanner III with CATS 1.3.4 version software. The experimental parameters like band size of the spot application, chamber saturation time, solvent front migration, slit width etc. were critically studied and optimum conditions were evolved. The drug was satisfactorily resolved with Rf value 0.66±0.03. The accuracy and repeatability of the proposed method was ascertained by evaluating various validation parameters like linearity (100 to 450 ng/spot), precision (intra-day RSD 0.17 to 0.69%, inter-day RSD 0.20 to 0.29%), accuracy (96.24±0.20), and specificity according to ICH guidelines. The limits of detection and quantification were 40ng/spot and 100ng/spot respectively. HPTLC method provides a faster and cost effective quantitative control for routine analysis of voglibose in its formulation.

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October 2019
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