Articles Accepted

STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF EMPAGLIFLOZIN AND LINAGLIPTIN IN PHARMACEUTICAL DOSAGE FORM
by Mr. T HEMANT KUMAR, 03 Jun 2019

A simple, specific, accurate, precise and stability indicating high performance liquid chromatographic method was developed and validated for the simultaneous estimation of Empagliflozin and Linagliptin using Thermo Scientific BDS C18 column (250 ×4.6 mm, 5 µm) with mobile phase consisting of Methanol: 0.1 % orthophosphoric acid in water, 45:55 v/v at a flow rate of 1 mL/min. Detection was carried out at 254 nm using UV detector. The retention time for Empagliflozin and Linagliptin were found to be 2.692 and 5.012 min respectively. The proposed method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Empagliflozin and Linagliptin were subjected to stress conditions of degradation including acidic, alkaline, oxidative, thermal and photolysis. The degradation studies indicated that Empagliflozin shown more degradation in all conditions except alkaline where as Linagliptin shown more degradation in acidic, alkaline and oxidative. This method can be successfully applicable for simultaneous Estimation of Empagliflozin and Linagliptin in bulk drugs and formulations.

Current Issue
November 2020
Quick Contact