Articles Accepted

METHOD DEVELOPMENT AND VALIDATION OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR ESTIMATION OF SELEXIPAG IN BULK DRUG AND DOSAGE FORM.
by Mr. Manjaramkar Dayanand Sambhajirao, 24 Apr 2020

The present paper describes new, simple, precise, accurate, development for estimation of Selexipag by RP-HPLC method. The described chromatographic method standardized using C18 column (Inertsil ODS-3 C18 Column 250x4.6mm,5 µ) and mobile phase containing 0.01M Ammonium formate Buffer (pH 4.5): Acetonitrile (15:85 V/V) at flow rate of 1mL/min the eluents were detected by DAD detector at 303 nm. The retention time was found to be 5.8. The system suitability parameters for Selexipag such as theoretical plates and tailing factor were found 15824 and 0.96 respectively. The linearity study of Selexipag was found in concentration range of 1 µg/mL- 3 µg/mL and correlation coefficient (r2) was found to be 0.9994 % recovery was found to be at each level was 98.77%-101.29%. % RSD values for Intraday and Interday Precision was found 0.51% & 0.386 %. The analytical method was validated and applied on marketed formulation.

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