Articles Accepted

DESIGN OF EXPERIMENT (DOE) BASED STUDY OF FACTORS AFFECTING THE UV ABSORPTION OF HYDROCHLOROTHIAZIDE
by Dr. Dr Lalit Kumar Tyagi, 29 Jul 2020

Current work was carried out to evaluate the effect of process variables on UV absorbance of Hydrochlorothiazide. The effect of three variables such as sample temperature, nature of the solvent and sample volume on UV absorption were studied by using design of experiments (DOE) approach. Central Composite design (CCD) was applied to investigate the effect of sample temperature (A) variation in the range of 15°C-45°C, nature of solvent (B) viz. ethanol, methanol and 4% Sodium Hydroxide and sample volume (C) in the range of 1.5 ml to 2 ml on UV absorbance of Hydrochlorothiazide. Each of the parameter was varied at three different levels (-1, 0, 1) and twenty suggested experimental runs were conducted with six central points. The data was then analyzed for model fitting and finding how each of the variables affected the response by using ANOVA. The three variables under study were found to have a moderate affect on the UV absorbance, as expected on the basis of theoretical concepts. However, the influence was found to be statistically insignificant as the P value for individual parameter was found to be greater than 0.05. Further the P value was less than 0.05 for the squares of temperature, solvent and combination of solvent and volume for UV absorbance of Hydrochlorothiazide, implying that these factors have a statistically significant bearing on UV absorbance. Results showed that out of the three parameters under study, the temperature of the sample affected the absorbance of Hydrochlorothiazide the most.

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