Articles Accepted

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD: APPLICATION FOR IN-VITRO DRUG RELEASE STUDIES OF SOLID LIPID NANOPARTICLES
by Ms. JAGRUTI B. PRAJAPATI, 04 Sep 2020

Rotigotine (RTG) loaded Solid Lipid Nanoparticles (SLNs) were developed for the treatment of Parkinson’s disease. To estimate RTG entrapped in SLNs, UV spectrophotometric method was developed and validated. The solvent and wavelength of detection were optimized in order to maximize sensitivity of proposed method. The method was validated for different parameters like Linearity, Precision, Specificity, Accuracy, Limit of Detection (LOD), Limit of Quantitation (LOQ) and Robustness as per ICH guidelines. A wavelength maximum of RTG in Phosphate Buffer Solution (PBS) 7.4 + 2% SLS mixture was found at 273 nm. The method was found to be linear in the range of 30 to 195 µg/mL with a correlation coefficient (r2) of 0.998. The accuracy of the method was studied by recovery study and % recovery was found in range of 98.34 to 100.38 %. The LOD and LOQ were found to be 1.6 µg/mL and 127.84 µg/mL respectively. The method is simple, accurate and requires relatively inexpensive instrument. The method was used successfully for determination of RTG loaded into SLNs.

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November 2020
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