Articles Accepted

RP-HPLC METHOD FOR THE ESTIMATION OF LORNOXICAM IN TABLETS AND IN DISSOLUTION SAMPLES USING MASS SPECTROMETRIC COMPATIBLE BUFFERS
by Dr. vijayalakshmi marella, 07 Aug 2019

A selective and sensitive reverse phase High Performance Liquid Chromatographic method has been developed and validated for the estimation of lornoxicam in bulk, pharmaceutical dosage forms and in dissolution samples. The analysis was performed isocratically on an inertsil column (250* 4.6mm, 5µm) using a mass spectrometric compatible mobile phase of 10mM ammonium acetate: acetonitrile (50:50v/v) at a flow rate of 1ml/min. The detection wavelength is 290nm.The retention time was found to be 4.573 min for lornoxicam. The linearity of the method has been satisfied with Beer Lambert’s law in the concentration range of 5-25µg/ml with a correlation coefficient of 0.9988.The mean recoveries assessed for lornoxicam were in the range of 100.39-101.86% indicating good accuracy of the method. The Limit of detection and limit of quantification were found to be 0.03 and 0.11µg/ml respectively. The developed method has been statistically validated in accordance with ICH guidelines and found to be mass spectrometric compatible, simple, precise, and accurate with the prescribed values. Thus the proposed method was successfully applied for the estimation of lornoxicam in routine quality control analysis of bulk, formulations and in dissolution samples.

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November 2020
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