Articles Accepted

SIMULTANEOUS ESTIMATION OF IMPURITIES IN MELATONIN BY AN HPLC METHOD COUPLED WITH DIODE ARRAY DETECTION
by Ms. shubhangi, 15 Dec 2019

In the present research work RP-HPLC method coupled with diode array detection for separation and quantitation of Impurity-I (2-(5-methoxy-1H-indol-3-yl) ethanamine), Impurity-II (3-(2-Aminoethyl)-1H-indol-5-ol) along with Melatonin was developed. Mobile phase containing 10 mMol/L Sodium dihydrogen phosphate: Acetonitrile (75:25 v/v) was found to give good resolution, effectively separating Melatonin and its Impurities. Calibration curve for Melatonin was found to be linear in the concentration range 2.5 to 7.5 μg/ ml. Calibration curve for impurity-I was found to be linear in concentration range 2.5 to 7.5 μg/ ml and the calibration curve for impurity –II was found to be linear in concentration range of 1.8 μg/ ml to 5.4μg/ ml. The percentage recovery estimated of Melatonin, Impurity-I, Impurity-II was found to be within 98.20 to 99.91, 97.42 to 104.04 , 98.35 to 100.06 respectively with R.S.D. The reported method is simple, precise, accurate and rapid for quantitation of Melatonin impurities along with Melatonin.

Current Issue
November 2020
Quick Contact