Articles Accepted

In vivo evaluation of curcumin revealed the biphasic angiogeneis activity in zebra fish experimental model
by Dr. Vandana, 23 Feb 2024

Objective: Curcumin is a major polyphenol compound found in Curcumin longa L. A wide spectrum of pharmacological and biological activities of curcumin have been reported, including antioxidant, antiprolofertive, anti-cancer, wound healing, antibacterial and so on. Owing to its hydrophobic nature, and poor bioavailability, curcumin is mainly solubilized with an amphiphilic solvent like dimethyl sulfoxide (DMSO) for in vitro or in vivo studies. The objective of the present study is to characterize curcumin solubilized in the solvent DMSO, and evaluate the angiogenesis activity of curcumin in zebrafish and chick chorioallantoic membrane assay. Methods: Curcumin was characterized using UV-VIS spectrophotometry and angiogenesis effect of curcumin dissolved in DMSO was evaluated using chick chorioallantoic assay (CAM) and zebrafish embryos. Results: The characterization of curcumin dissolved in DMSO with UV-VIS spectrophotometry showed the hypschomatic shift in wavelength maximum from 425 nm to 347 nm, indicating solvent-curcumin interaction. Evaluation of angiogenesis activity in the chick chorioallantoic membrane model and zebrafish embryos demonstrated dose-dependent promotion of angiogenesis. Conclusions: The biphasic, dose-dependent angiogenesis feature of curcumin confirmed in CAM and zebrafish models. Moreover, the use of the zebrafish model for efficacy studies like angiogenesis evaluation further corroborated. The solvent-curcumin interactions, change of molecular properties, and their therapeutic consequences need further investigation.

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