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RP-HPLC AND HPTLC METHODS FOR THE ESTIMATION OF LUPEOL IN. HEMIDESMUS INDICUS LINN. ROOT EXTRACTS AND POLYHERBAL FORMULATION USING STANDARD MA

Rajendran Jayaprakasama , Chacko Anita Elizabetha , Thomas Tresaa , Johnson Neethua and Thengungal Kochupapy Ravia

a Department of Pharmaceutical Analysis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore - 641 044. Tamilnadu, India

* For Correspondence Email: jprcognosy@gmail.com


ABSTRACT

The roots of Hemidesmus indicus, belonging to the family Ascelpidaceae, are used as blood purifier, antileucorrhoeic, galactogenic, antidiarrhoeal, antirheumatic, antisyphilitic, febrifuge and possesses chemical constituents like triterpenoids (lupeol), flavonoids, glycosides and tannins. Roots were dried and extracted with petroleum ether, chloroform and methanol by using Soxhlet apparatus. Two simple and sensitive chromatographic methods, namely, HPTLC and HPLC, were developed for determination of lupeol from the extract of H. indicus and its polyherbal formulation and validated according to ICH guidelines. The HPTLC method linear regression data for the standard lupeol a concentration range of 400-900ng/spot and correlation coefficient (r) was 0.9904. The HPLC calibration curves of standard lupeol showed good linearity range from 20 to 100μg/ml and correlation coefficient (r) was 0.9929. The HPTLC and HPLC methods are simple, precise, accurate and specific. Hence, these methods can be used for the standardization of lupeol in herbal plants and polyherbal formulations.

Year 2020 | Volume No. 57 | Issue No.7 | Page No. 40-46
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