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DEVELOPMENT AND VALIDATION OF A CHEMOMETRICS ASSISTED SPECTROSCOPIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF GALLIC ACID, ELLAGIC ACID AND CURCUMIN IN POLYHERBAL ANTIDIABETIC FORMULATIONS

Shah M. A. a,b* , Patel H. U. c and Raj H.A. d

a Gujarat Technological University, Ahmedabad 382 424, Gujarat, India

b Department of Quality Assurance, ROFEL, Shri G.M Bilakhia College of Pharmacy, Vapi -396 191, Gujarat, India

c Shri Satsangi Saketdham “Ram Ashram”, Group of Institutes, Faculty of Pharmacy, Vadasma, Mehsana - 382 730, Gujarat, India

d Laxminarayan Dev College of Pharmacy, Bholav, Bharuch - 392 001, Gujarat, India

*For correspondence E-mail: megha.pharmacist@gmail.com


ABSTRACT

Two chemometric methods, Inverse Least Square (ILS) and Classical Least Square (CLS), were applied for the simultaneous estimation of gallic acid, ellagic acid and curcumin in polyherbal antidiabetic formulation. Twenty mixed solutions were prepared for the chemometric calibration as training set and 10 mixed solutions were prepared as validation set. The absorbance data matrix was obtained by measuring the absorbance at 20 different wavelengths, from 241 to 279 nm with the interval of 2 nm (Δλ= 2 nm). The developed calibrations were successfully tested for three antidiabetic polyherbal formulations for their gallic acid, ellagic acid and curcumin contents. Developed methods were validated and root mean square error of precision (RMSEP) was determined. Both chemometric methods in this study can be satisfactorily used for the quantitative analysis in polyherbal dosage forms. The chemometric calculations were performed by using the chemometrics toolbox with MATLAB R2015a software.

Year 2019 | Volume No. 56 | Issue No.06 | Page No. 67-73
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