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STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF PIOGLITAZONE, AMLODIPINE, ATORVASTATIN AND CLOPIDOGREL

Monikaa, Pandey S.b and Chawla P.a,c*

a Department of Pharmaceutical Chemistry, Babu Banarasi Das University, Faizabad Road, Lucknow - 226 028, Uttar Pradesh, India

b Department of Pharmacy, Sarojini Naidu Medical College, Agra - 282 003, Uttar Pradesh, India

c Department of Pharmaceutical Chemistry, ISF College of Pharmacy, Moga - 142001, Punjab, India

*For Correspondence: E-mail: pvchawla@gmail.com


ABSTRACT

A new simple, rapid, isocratic and stability indicating reverse phase high performance liquid chromatography (HPLC) method has been developed for simultaneous estimation of pioglitazone (P), amlodipine (AM), atorvastatin (AT) and clopidogrel (CP). Isocratic separation was achieved on Millennium M Sil C18 (125 mm ´4.6 mm) column, with mobile phase comprising of methanol: 0.02 M KH2PO4 (55:45 V/V), pH adjusted 4.0 with 0.01% ortho phosphoric acid. The flow rate was maintained 1mL/min and analytes were screened with U.V. detector. The method was validated according to ICH guidelines with respect to linearity, accuracy, precision and specificity. The drugs were exposed to various stress conditions like acidic and basic hydrolysis, oxidation, exposure to UV light and temperature (dry heat) and the stressed sample were analysed by the proposed method. No co-eluting, interfering peaks from excipients, impurities were observed during stress condition and all the degraded peaks are well resolved from parent peaks.

Year 2019 | Volume No. 56 | Issue No.07 | Page No. 50-58
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