Article Details

SIMULTANEOUS DETERMINATION OF DOMPERIDONE AND PARABEN PRESERVATIVE IN ORAL FORMULATION: REVERSED-PHASE LIQUID CHROMATOGRAPHIC METHOD

Patel A.a, Firke S. D.*b, Patil R. R.b,

Kalaskar M. G.b, Bari S. B.a and Surana S. J.b,

a Department of Pharmaceutical Analysis, H. R. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule - 425 405, Maharashtra, India

b Department of Pharmaceutical Analysis, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule - 425 405, Maharashtra, India

For Correspondcence: Email: sandipfirke@rediffmail.com


ABSTRACT

A novel RP-HPLC method was developed and validated for the determination of compounds in an oral solution. The method describes the determination of domperidone along with sodium methylparaben, sodium propylparaben and sodium benzoate in liquid oral formulation. The chromatographic separations were performed using BDS Hypersil 5 μm C18 column and gradient elution (solvent A: phosphate buffer, pH 3.5 and solvent B: methanol) keeping a flow rate of 1.5 mL/min. Detection was done at dual wavelength (232 nm for domperidone and sodium benzoate, and 257 nm for sodium methylparaben and sodium propylparaben). Analysis time was < 17 min. The retention times for domperidone, sodium benzoate, sodium methylparaben and sodium propylparaben were found to be 10.0, 6.5, 8.0, and 13.5 min, The calibration curves for domperidone, sodium benzoate, sodium methylparaben and sodium propylparaben were found to be linear in the range of 250-750, 50-150, 50-150, and 5-15 μg/mL, respectively.

Year 2019 | Volume No. 56 | Issue No.08 | Page No. 81-83
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