ABSTRACT

The primary objective of this study was to assess the antimicrobial activities of various polar and nonpolar solvent extracts derived from the leaves of Viola odorata. The disc diffusion method was employed to test the antimicrobial susceptibility against a diverse range of pathogenic microorganisms, including Escherichia coli, Erwinia carotovora, Klebsiella pneumoniae, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus and the fungus Candida albicans. The results indicated that isobutanol and ethyl acetate fractions exhibited significantly higher antimicrobial activity compared to petroleum ether, crude, chloroform and aqueous fractions. Among the tested bacteria, E. carotovora was the most susceptible bacterium to all the extracts of V. odorata, whereas S. typhi demonstrated the highest resistance. The isobutanol extract inhibited the proliferation of E. carotovora by 62 % at a concentration of 2 mg disc-1, while the ethyl acetate extract achieved 53% inhibition under the same conditions. Additionally, our findings revealed that the overall antimicrobial activity of V. odorata leaves was consistently effective against both Gram-positive and Gram-negative bacterial strains.