Articles Accepted

Apoptosis induction of ethanolic extract of Amomum subulatum in HeLa cell lines
by Dr. Ramakrishnan Prakash, 02 Nov 2018

The defective apoptosis is believed to play a vital role in survival and proliferation of cancer cells. Hence, the provoke of apoptosis in cancer cells is one of the targets for anticancer treatment. The main objective of the current study is to investigate the anticancer property of ethanolic extract of Amomum subulatum against HeLa cell line. The extract cytotoxicity was assessed by 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay, where the IC50 value was used as a parameter for cytotoxicity. The ethanolic extract that showed anticarcinogenic properties in HeLa cells had an IC50 of 62.5 μg/ml, IC50 value of 5 Fluorouracil was found to be 7.8 μg/ml. The apoptotic morphological changes in HeLa cells were observed were observed using an inverted microscope and showed chromatin condensation, cell shrinkage. Following acridine orange and ethidium bromide staining, treatment with 62.5 μg/ml of ethanolic extracts caused typical apoptotic morphological changes in HeLa cells. Molecular analysis of DNA fragmentation was used to examine intrinsic apoptosis induced by the ethanolic extracts. These results showed a typical intrinsic apoptotic characterisation, which included fragmentation of nuclear DNA in ethanolic extract-treated HeLa cells. We also found that the expression of Bcl-2 levels were downregulated and upregulated p53 mRNA levels. Overall, the results of the present study suggest that ethanolic extract of Amomum subulatum may prove beneficial in the management of cervical cancer.

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