Articles Accepted

Development and validation of analytical method for simultaneous estimation of Cilnidipine, Chlorthalidone and Telmisartan in bulk and tablet dosage form
by Mrs. swapna.goday, 22 Feb 2019

A simple, accurate and precise method was developed for the simultaneous estimation of cilnidipine, chlorthalidone and telmisartan in tablet dosage form on Agilent C18 column (150*4.6mm, 5µ) using buffer and acetonitrile in the ratio of 55:45 was pumped through a column with a flow rate of 1ml/min. Buffer used in this method was 0.1% ortho phosphoric acid at a pH of 2.5. The run time was 5 min. and the retention time of cilnidipine, chlorthalidone and telmisartan were found to be 2.105min, 2.813min and 3.505min. Reference standard solutions of the drugs were prepared by dissolving firstly in methanol and then diluted with methanol and water (50:50). This method provides good linearity ( R2=0.999) over a range of 5-30 µg/ml of cilnidipine, 6.25-37.5 µg/ml of chlorthalidone and 20-120 µg/ml of telmisartan. Optimized wavelength was 238nm. The method was validated in terms of specificity, linearity, accuracy, precision, detection limit, quantification limit, robustness and forced degradation.

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