Articles Accepted

SIMULTANEOUS ESTIMATION OF DOXORUBICIN ANDHYDROCHAVICOL IN BULK AND PHARMACEUTICAL FORMULATION BY Q-ABSORPTION RATIO METHOD
by Dr. Sachin Shivling Bhusari, 11 May 2019

A simple, rapid, accurate, precise and economical UV-spectrophotometric method has been developed and validated for simultaneous estimation of DOXO and OH-CHV in a Pharmaceutical formulation. It is based on the Q-analysis UV Spectrophotometric method. The absorption spectra of DOXO and OH-CHV were obtained over the range of 200-800 nm, and the absorption maxima were determined. Multiple calibration standards were prepared for both the drugs separately and absorbance were recorded at respective absorption maxima. Calibration curve were plotted and the linear responses were studied. Various analytical method validation parameters viz. accuracy, precision, LOD, LOQ, robustness and ruggedness were calculated using quality control standards. The absorbance maxima of DOXO and OH-CHV were found to be 477 nm and 284 nm, respectively in methanol. In Q-analysis, the isoabsorptive point for both the drugs was found at 297 nm. The linearity range was between 5-40 μg/ml for DOXO and 5-40 μg/ml for OH-CHV at their respective wavelengths. The intra-day and inter-day study shows percent relative standard deviation in the range of 0.9991 to 0.9993. LOD and LOQ were found to be 0.024 µg/ml and 0.075µg/ml for DOXO whereas 0.228μg/ml and 0.692μg/ml for OH-CHV. The total percent recoveries of DOXO and OH-CHV were found to be 98.75 and 99.41 respectively.

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October 2019
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