Articles Accepted

Green synthesis of gold nanoparticles of isolated citrus bioflavonoid from orange: Characterization and in vitro cytotoxicity against colon cancer cellines COLO 320DM and HT29
by Mr. dheeraj, 02 May 2020

The aim of present research was to carryout green synthesis of Gold nanoparticles of isolated citrus bioflavonoids from Citrus sinensis (orange) peel extract using soxhlet extraction method and column chromatography and evaluated its anticancer potential. The methanolic extract of orange peel was obtained by soxhlet extraction method and citrus bioflavonoid isolated by using column chromatography. The gold nanoparticles developed by green synthesis method as 1 % aqueous solution of isolated citrus bioflavonoid were mixed with 1 % solutions of HAuCl4 solutions and incubated at ambient temperature for 4 to 5 hours and observed the color change for development of nanoparticles, these nanoparticles of gold were evaluated by UV visible spectra, FTIR, SEM, XRD, analysis and further evaluated for antimicrobial potential against E coli, staph aureus and pseudomonas aeruginosa. The cytotoxicity study was carried out by in vitro assay like MTT, SRB and tryphan blue assay method against colon cancer cell line Colo 320 DM, and HT 29,. The SEM showed that nanosized particles 80 -100nm were formed whereas no antimicrobial potential was observed in all developed nanoparticles the cytotoxicity results revealed that CBFGNP against Colo320 DM showed 72.28% inhibition while pure CBF shows 70.46% inhibition. Against HT 29 CBFGNP shows 69.79% inhibition by MTT assay and SRB Assay showed 80.15% and 58.29% inhibition respectively for Colo320 DM and HT29 further in tryphan blue assay CBFGNP showed that 90.29% and 85% non viability against Colo320 DM and HT29. The results conclude that Gold nanoparticles of citrus bioflavonoid (CBFGNP) showed more cytotoxicity against Colo320 DM and HT29 as compared with pure citrus bioflavonoid when assessed by three different in vitro cytotoxicity assay.

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