Articles Accepted

Nephroprotective effect of Tukhm-e-Karafs (Apium graveolens L.) against Gentamicin induced nephrotoxicity in experimental animals
by Dr. Mohammad Zakir, 05 Jan 2020

Tukhm-e Karafs is the seed of Apium graveolens L. which is an important aromatic plant grown globally as fresh herb used as salad crop. This seed possesses potential pharmacological activity like hepatoprotective, diuretic, lithotriptic etc. It is used as single drug or major constituents in different polyherbal Unani formulations to treat various ailments. The present study was designed to evaluate Nephroprotective effect of Tukhm-e Karafs against Gentamicin (80 mg/kg bw) induced nephrotoxicity in male Wistar rats. The effect was tested at two dose levels i.e. 500 mg/kg and 1000 mg/kg BW (calculated based on clinical dose mentioned in Unani literature). The study was divided in two models i.e curative model where toxicity was induced using Gentamicin prior to administration of test drug, whereas in protective model toxicant (Gentamicin) and test drug was given parallel. Both models consist of four groups of six animals in each group included control group, negative control and two test dose group. The efficacy was determined based on estimation of biochemical parameters (creatinine, uric acid, BUN and serum electrolytes) along with quantification of kidney injury molecule-1 (KIM-1) using ELISA method in kidney tissue homogenate and histopathological study of kidney tissue. The findings of the study showed that Tukhm-e Karafs at both tested dose showed prominent reduction of biochemical parameter as compared to negative control group in both models. KIM-1 reduction was observed but not found statistically significant in both models. Histopathological changes showed that drugs have inhibited Gentamicin induced injury in curative group as well as protective group. These results concluded Nephroprotective effect of Tukhm-e Karafs.

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