Articles Accepted

Abstract Background: The SARS-CoV-2 has posed a serious threat to mankind, affecting millions of people worldwide. A traditional Ayurvedic formulation; Ayush Kwath Churna, known for its immunity-boosting properties, has gained attention during the pandemic. However, concerns regarding the adulteration of this formulation have raised significant issues related to its quality, safety, and efficacy. Aim: For the purpose of standardization, the current study set out to create a straightforward, precise, and sensitive H.P.T.L.C. method for the detection and measurement of the alkaloid marker compound eugenol in the Ayurvedic formulation Ayush Kwath Churna. Materials and methods: HPTLC study was done with methanolic extraction Ayush Kwath Churna (AKC) using Eugenol as a marker compound. The study was performed over readymade plates using optimized mobile phase; toluene: chlorofom: methnol: formic acid (4:4:1:0.3) and estimation of amount of Eugenol was performed at lambdamax 282nm. The ICH guideline Q2 (R1), which addresses parameters including linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ), was followed in order to validate the method. With an Rf value of around 0.4 ± 0.018, the calibration curve for eugenol showed linearity in the range of 1–7 µg/spot. The results showed that the LOD was 0.13 µg/spot and the LOQ was 0.40 µg/spot. Relative standard deviations (RSD) of less than 2% demonstrated the method's great repeatability. With recovery rates of 97.13%, recovery studies showed good accuracy. Results: The Rf of marker compound eugenol was observed at 0.4 ± 0.018 and presence of eugenol in ayush kwath churna formulation was qualified at lambdamax 282 nm. Conclusion: Eugenol in Ayush Kwath Churna can be properly standardized and princely analyzed using this procedure. Furthermore, it may assist researchers in elucidating the significant role of eugenol present in the formulation, thereby contributing to the understanding of its therapeutic potential.