ABSTRACT

A simple, precise, rapid, accurate and economic reverse phase high performance liquid chromatographic method has been developed for the estimation of mebeverine hydrochloride in bulk and tablet dosage form. The separation was achieved by using kinetex column C18 (150×4.6mm, 5µ) and 15mM ammonium acetate and methanol in proportion of 30:70 v/v as mobile phase, at a flow rate of 1.0mL/min. The detection was carried out at 230nm. The retention time of mebeverine was found to be 3.346 minutes.The limit of detection and limit of quantitation were found to be 0.005µg/mL and 0.016µg/mL, respectively. The reliability of the proposed method was ascertained by evaluating various validation parameters like linearity, precision, accuracy and specificity according to ICH guidelines. The proposed method applies LC-MS compatible buffers which increases the life time of column and can be used as lc-ms compatible method. This method acts as a quality control tool for routine analysis of mebeverine hydrochloride in bulk and tablet dosage forms.