ABSTRACT

A new method has been developed and validated for the simultaneous estimation of gallic acid and eugenol for the poly-herbal formulation. The HP-TLC (High Performance Thin Layer Chromatography) method was developed using pre-coated silica gel 60 F₂₅₄ on aluminum plate and the solvent system used was iso propyl alcohol : hexane: ethyl acetate: glacial acetic acid (50:30:30:1 V/V) and scanned at 254 nm wavelength. The R_f value for gallic acid was 0.608±0.041 and 0.752±0.035 for eugenol. The range of linearity detected for gallic acid and eugenol was 2-10 ng µL^-1. The method was validated in accordance with ICH (International Council for Harmonization) guidelines for linearity, range, LOD, LOQ, precision, specificity and robustness. The developed method was found to be easy, simple, selective, specific and robust.