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Development and Validation of Difference Spectroscopic Method for the Estimation ofTolperisone Hcl in Bulk and Pharmaceutical Dosage Form

Gohil K., Shah B., Patel B., Patel P. and Patel N.


Difference spectroscopic method is based on the principle that tolperisone hydrochloride can exhibit twodifferent absorption spectra in basic and acidic medium. A stock solution (1 mg/ml) was prepared withdistilled water. Further dilution was made by using 0.1 N sodium hydroxide and 0.1 N hydrochloric acidseparately. The maxima and minima in the difference spectra of tolperisone hydrochloride were at 231nmand 263nm, respectively. Difference in absorbance between these maxima and minima was calculatedto find out the amplitude. This amplitude was plotted against concentration. Linearity was found in theconcentration range of 10-50?g/ml. The LOD and LOQ of tolperisone hydrochloride were found to be0.064?g/mL and 0.196?g/mL respectively. The percentage recovery study of the drug for the proposedmethod was found in the range of 99.31 - 99.94% for tolperisone hydrochloride. The proposed methodis recommended for routine analysis since it is rapid, simple, precise and accurate.
Year 2013 | Volume No. 50 | Issue No.1 | Page No. 59
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