ABSTRACT

Marker compounds play an important role in standardization of herbal extracts and herbal formulations. The isolation of marker/s from crude extract or fractions of an extract is often a lengthy and expensive process and it many times requires numerous separation steps involving use of different separation techniques. The present work describes new method of isolation of betaine, a chemical marker from the plant Achyranthes aspera by preparative TLC and its characterization by chemical and spectroscopic techniques like IR, NMR and mass spectroscopy.