Article Details

RP-HPLC METHOD DEVELOPMENT FOR THE ASSAY OF AMPHOTERICIN B

Mohankrishna L.*, Reddy P. J., Reddy.B. P. and Navya P.

Vignan Institute of Pharmaceutical Sciences Deshmukhi, Nalgonda, Andhra Pradesh - 508284, India. *E-mail: mohanlokireddy@gmail.com


ABSTRACT

A sensitive and precise HPLC procedure has been developed for the assay of amphotericin B in bulksamples and pharmaceutical formulations by using a C18 column [Kromosil, C18, (5 ?m, 4.6mm x 250mm; Make. Waters)], and mobile phase combination is 1% formic acid in water and acetonitrile in ratioof 45:55 V/V. The procedure has been validated as per the ICH guidelines. The ? max of detection wasfixed at 407 nm, so that there was less interference from mobile phase with highest sensitivity accordingto UV analysis. Calibration plots were linear in the range of 10-100 ?g/mL and the LOD and LOQ were0.02 ?g/mL and 0.06 ?g/mL respectively. The high recovery and low relative standard deviation confirmthe suitability of the method for routine quality control determination of amphotericin B in differentformulations.
Year 2014 | Volume No. 51 | Issue No.02 | Page No. 16-20
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