Article Details

ISOLATION AND CHARACTERIZATION OF EXOPOLYSACCHARIDES FROM LACTIC ACID BACTERIA AND ITS USE IN FORMULATION DEVELOPMENT

Gadad A. P.*, Singh R., Darbha N. S. and Dandagi P. M.

Department of Pharmaceutics KLEUs College of Pharmacy Belgaum-590010, Karnataka, India *E-mail: gadadap@rediffmail.com


ABSTRACT

Exopolysaccharides (EPS) are polymers that consist principally of carbohydrates and are excreted bysome microorganisms onto the outside of their cell walls. Several strains of lactic acid bacteria (LAB) areable to produce EPS which can be used as natural additives to enhance the rheology and texture of novelfood products. they are considered as food-grade microorganisms known as Generally Recognizedas Safe (GRAS). Various milk and curd samples were screened for the isolation of lactobacilli. Thecolonies were subcultured in milk media (50%) maintained at 20 C for 72 hours under anaerobicconditions for the production of EPS. EPS was then isolated after ethanol precipitation. Two promisingEPS were obtained from two different milk samples and named as EPS 1 and EPS 5. The two EPS wereisolated showing promising results. The isolated EPS were evaluated as an excipient in the formulationdevelopment and were explored as binder in tablets. The tablets were evaluated for hardness, friability,weight uniformity, disintegration and invitro dissolution profiles. The binding property of the two EPS wasinvestigated in diclofenac tablets formulated by wet granulation using polyvinylpyrrolidone (PVP) andgelatin. Results indicated that formulation prepared with EPS have shown good hardness and friabilityin tablet formulations when compared to PVP and gelatin.
Year 2014 | Volume No. 51 | Issue No.02 | Page No. 39-46
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