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A Validated RP-HPLC Method for the Simultaneous Estimation of Pantoprazole and Cinitapride in A Pharmaceutical Formulation

Dighade N. R.*, Padmane S. P. and Kasture A. V.

Adv. V. R. Manohar Institute of Diploma in Pharmacy Wanadongri, Hingna Road, Nagpur441110 Maharashtra, India. *E-mail:


The study describes a validated stability indicating reverse- phase HPLC method for the simultaneousestimation of pantoprazole and cinitapride in capsule formulation. The proposed RP-HPLC methodutilizes an Eclipse XDB C18 Column (150 4.6 mm i.d., 5?m), optimum mobile phase consisting of 10mM phosphate buffer: acetonitrile: THF in the ratio of 64:36:0.5 V/V (pH 3.5) V/V, effluent flow rate1 mL/min and UV detection wavelength of 266 nm. The selected chromatographic conditions werefound to effectively separate pantoprazole and cinitapride with retention time of 7.17 min and 3.56 min,respectively. Linearity for pantoprazole and cinitapride was found in the range of 2-30 ?g/mL and 0.15-2.28 ?g/mL, respectively. The developed method was statistically validated for the linearity, accuracy,precision, robustness, ruggedness and specificity. The proposed method was found to be simple,accurate, precise, economical and specific. Therefore, it can be used for simultaneous analysis of thesedrugs in capsule formulation.
Year 2014 | Volume No. 51 | Issue No.01 | Page No. 27-33
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